University of Southern California

Research

Cell Culture & Analysis

cell culture mouse stem cells comp

Bioreagent and Cell Culture Core

The Norris Cancer Center Bioreagent and Cell Culture Core supports Southern California Clinical and Translational Science Institute (SC CTSI) in vitro cancer-related research. Equipment and services include: preparation of reagents for cell culture and bioreagents. The Core has acquired a New Brunswick BioReactor and is in the process of developing and evaluating lipofectin preparations for cell transfection.

Health Sciences Campus
USC/Norris Cancer Center
1303 Mission Road
Los Angeles, CA 90033
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Center for Liver Disease (Cell Culture)

The Cell Separation and Culture Core of the USC Research Center for Liver Diseases is located on the 4th and 8th floors of the Hoffman Research Building. Services include: the Cell Culture component of the Core provides isolation of mouse and rat hepatocytes from normal and diseased livers, cell line banking, and human hepatocytes. The Cell Separation component of the Core provides isolation of cell subtypes for cell function analysis. Equipment includes: incubators, laminar flow hoods, microscopes, liquid nitrogen storage, a Miltenyi Biotec AutoMACS Pro Separator and a BD FACSVerse Flow Cytometer. Priority is given to Liver Center members at USC.

Health Sciences Campus
Elaine Stevely Hoffman Medical Research Center, 4th floor
2012 Zonal Avenue, HMR 101
Los Angeles, CA 90034
Cell Culture component: shellylu@usc.edu
Cell Separation component: zxliu@usc.edu

Choi Family Therapeutic Screening Facility

The Choi Family Therapeutic Screening Facility enables investigators from USC and beyond to improve their understanding of diseases and accelerate the discovery of potential therapeutic drugs. Equipment and services include: extensive chemical libraries, state-of-the-art screening equipment and trained technicians.

Health Sciences Campus
1425 San Pablo Street, BCC 205A
Los Angeles, CA 90033
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Circulating Tumor Cells Research Core

Circulating tumor cells (CTCs) are cancer cells shed by solid tumors into the bloodstream. CTCs have recently emerged as valuable prognostic and predictive cancer biomarkers, providing a non-invasive window into disease biology and progression that can be sampled repeatedly over time from a simple blood draw. Moreover, CTC sampling enables real time tracking of cancer plasticity as tumor cells evolve over time and through progressive lines of therapy, thus elucidating mechanisms of cancer dissemination and identifying new therapeutic targets. As such, CTC characterization holds the promise of enabling real-time molecular phenotyping of individual cancer patients’ tumors at diagnosis and throughout treatment, advancing precision medicine in this important and vast patient group. The USC Norris CTC Research Core is a state-of-the-art, multi-platform facility for the capture and analysis of peripheral blood CTCs. The Core employs a variety of technologies that enrich CTCs for enumeration or molecular characterization. Enrichment is done from blood samples drawn by standard venous puncture from patients. In addition, Dr. Goldkorn’s team has developed mouse xenograft research models using labeled CTCs that can be recovered from the mice.

Health Sciences Campus
USC Norris Cancer Center
1450 Biggy Street., NRT6516
Los Angeles, CA 90033
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Non-Parenchymal Liver Cell Core & Animal Core

The Research Center for Alcoholic Liver and Pancreatic Disease core laboratories supports center investigators and serves as a national resource. Animal Core services include: mouse intragastric (iG) and ad lib ethanol feeding models which produce clinically relevant pathologic spectra of ALPD; routine BAL and ALT measurements; non-parenchymal liver cell core services, terminal experiments and sample collection, as well as a Tissue Sharing and Training Program. Animal models include: Rodent iG Models Standard iG model; Hybrid model of Western Diet ad lib and Alcohol iG feeding for ASH with liver fibrosis; Hybrid plus Binge Model of Alcoholic Hepatitis and Alcohol/HCV Liver Tumor Models: Metabolomics Core services include: metabolomics analysis in animals and isolated cells, such as stable isotope-based (13C and 2H).

Health Sciences Campus
Mudd Memorial Research Building
1333 San Pablo Street, MMR 412
Los Angeles, CA
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Stem Cell Core

The Stem Cell Core in the Saban Research Institute at CHLA is a shared lab and centralized support facility funded by the California Institute for Regenerative Medicine. Services include: cell distribution, biobanking, repository and stem cell testing, assisting both CHLA investigators and non-hospital scientists with their research. Equipment includes: two shared laboratory spaces, imaging equipment, real-time PCR, nuclear transfection, flow cytometry and long term storage.

Children’s Hospital Los Angeles
Saban Institute Small Animal Imaging Research Center
4661 Sunset Blvd.
Los Angeles, CA 90027
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Stem Cell Core

The USC Stem Cell Core facility is a CIRM-funded comprehensive support center which provides knowledge, resources and state of the art technology to researchers working with stem cells. Services include: stem cell and biomedical research and centralized technologies and technical assistance to facilitate pluripotent stem cell research. The facility also offers courses and workshops focused on human pluripotent stem cell culture and research methodologies.

Health Sciences Campus
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell
1425 San Pablo Street
Los Angeles, California 90033

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