University of Southern California

Research

Cell Culture & Analysis

cell culture mouse stem cells comp

Aging Biomarker and Service Core

Aging Biomarker and Service Core owns QuickPlex SQ 120 (Meso Scale Discovery, MSD) and SpectraMax M3 multi-mode microplate readers (Molecular Device). The ELISA services include 1) cytokines measurement, 2) Alzheimer-related markers (Amyloid beta, phospho-tau) measurement, 3) hormones measurement including GH, human and mouse IGF-I& IGFBPs, etc., 4) metabolic biomarkers measurement including insulin, leptin and adiponectin, 5) mitochondrial derived peptides (MDPs) measurement including humanin and the other novel MDPs, 6) new assay development.

University Park Campus
3715 McClintock Ave.
Davis School of Gerontology
GER #B1B
Los Angeles, CA 90089
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Bioreagent and Cell Culture Core

The Norris Cancer Center Bioreagent and Cell Culture Core supports Southern California Clinical and Translational Science Institute (SC CTSI) in vitro cancer-related research. Equipment and services include: preparation of reagents for cell culture and bioreagents. The Core has acquired a New Brunswick BioReactor and is in the process of developing and evaluating lipofectin preparations for cell transfection.

Health Sciences Campus
USC/Norris Cancer Center
1303 Mission Road
Los Angeles, CA 90033
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Chang Stem Cell Engineering Facility

The Chang Stem Cell Engineering Facility genetically modifies embryonic stem (ES) cells and induced pluripotent stem (iPS) cell lines. Genetically modified ES and iPS cell lines derived from disease-specific animal models and patients provide an opportunity to investigate the mechanisms of disease and to further develop new treatments for clinical therapy. Services include: genome editing, iPS cell derivation, and ES cell derivation.

Health Sciences Campus
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC
1425 San Pablo Street, BCC 205
Los Angeles, CA 90033
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Choi Family Therapeutic Screening Facility

The Choi Family Therapeutic Screening Facility enables investigators from USC and beyond to improve their understanding of diseases and accelerate the discovery of potential therapeutic drugs. Equipment and services include: extensive chemical libraries, state-of-the-art screening equipment and trained technicians.

Health Sciences Campus
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC
1425 San Pablo Street, BCC 205A
Los Angeles, CA 90033
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Circulating Tumor Cell / Liquid Biopsy Research Core

Circulating tumor cells (CTCs) are cancer cells shed by solid tumors into the bloodstream. CTCs have recently emerged as valuable prognostic and predictive cancer biomarkers, providing a non-invasive window into disease biology and progression that can be sampled repeatedly over time from a simple blood draw. Moreover, CTC sampling enables real time tracking of cancer plasticity as tumor cells evolve over time and through progressive lines of therapy, thus elucidating mechanisms of cancer dissemination and identifying new therapeutic targets. As such, CTC characterization holds the promise of enabling real-time molecular phenotyping of individual cancer patients’ tumors at diagnosis and throughout treatment, advancing precision medicine in this important and vast patient group. The USC Norris CTC Research Core is a state-of-the-art, multi-platform facility for the capture and analysis of peripheral blood CTCs. The Core employs a variety of technologies that enrich CTCs for enumeration or molecular characterization. Enrichment is done from blood samples drawn by standard venous puncture from patients. In addition, Dr. Goldkorn’s team has developed mouse xenograft research models using labeled CTCs that can be recovered from the mice.

Health Sciences Campus
USC Norris Cancer Center
1450 Biggy Street., NRT6516
Los Angeles, CA 90033
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CIRM Stem Cell Core Facility

The CIRM Stem Cell Core Facility is a CIRM-funded comprehensive support center that provides knowledge, resources and state-of-the-art technology to researchers working with stem cells. Services include: stem cell and biomedical research and centralized technologies and technical assistance to facilitate pluripotent stem cell research. The facility also offers courses and workshops focused on human pluripotent stem cell culture and research methodologies.

Health Sciences Campus
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC
1425 San Pablo Street, BCC 209
Los Angeles, CA 90033

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Integrative Liver Cell Core

The Research Center for Alcoholic Liver and Pancreatic Disease core laboratories supports center investigators and serves as a national resource. Animal Core services include: mouse intragastric (iG) and ad lib ethanol feeding models which produce clinically relevant pathologic spectra of ALPD; routine BAL and ALT measurements; non-parenchymal liver cell core services, terminal experiments and sample collection, as well as a Tissue Sharing and Training Program. Animal models include: Rodent iG Models Standard iG model; Hybrid model of Western Diet ad lib and Alcohol iG feeding for ASH with liver fibrosis; Hybrid plus Binge Model of Alcoholic Hepatitis and Alcohol/HCV Liver Tumor Models: Metabolomics Core services include: metabolomics analysis in animals and isolated cells, such as stable isotope-based (13C and 2H).

Health Sciences Campus
Mudd Memorial Research Building
1333 San Pablo Street, MMR 412
Los Angeles, CA
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Seahorse Core Facility Gerontology

About the Seahorse XF96 Analyzer: The Seahorse XF96 is a unique instrument that measure extracellular O2, pH, glucose and lactate fluxes to provide estimates of glucose utilization in cultured cells. The XF96 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways. Unlike radiometric assays, the XF96 does not require radiolabeled substrates, and unlike measurements of cellular oxygen consumption using the classic Clark electrode, it is possible to make high throughput measurements on 96 samples simultaneously in a microplate format.

Davis School of Gerontology
3715 McClintok Ave, B1A
Los Angeles, CA 90089
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Stem Cell Core

The Stem Cell Core in the Saban Research Institute at CHLA is a shared lab and centralized support facility funded by the California Institute for Regenerative Medicine. Services include: cell distribution, biobanking, repository and stem cell testing, assisting both CHLA investigators and non-hospital scientists with their research. Equipment includes: two shared laboratory spaces, imaging equipment, real-time PCR, nuclear transfection, flow cytometry and long term storage.

Children’s Hospital Los Angeles
Saban Institute Small Animal Imaging Research Center
4661 Sunset Blvd.
Los Angeles, CA 90027
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